Solution for determining specificity of donor and / or recipient tissues by the NGS method when creating bone marrow donor registries.
GeneValue™ HLA Solution
For research use only
Kit includes library prep reagents and access to GeneValue™ HLA Software for all data analysis and reporting stages
This set allows the qualitative determination of alleles of HLA genes (HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1) in 3-field resolution to allow the detection of new alleles.
Product highlights
Preparation of 192 samples in one day
Genotype definition in 3-field resolution
Fully automatic data analysis and quality control
Identification of new alleles
Reduction of quantification and normalisation steps for samples and libraries
HLA antigens play an important role in regulating the immune response to foreign antigens, determining the uniqueness of a person's tissue type and controlling the rejection of foreign tissues during transplantation.
In a clinical setting, HLA genotyping is used to determine the tissue specificity of the donor and recipient to select a histocompatible pair for allogeneic organ or tissue transplantation.
The most common need for finding a donor is to obtain hematopoietic stem cells (HSC) for red bone marrow (RBM) transplantation. HSC transplantation is an effective method for treating oncohaematological and other oncological and hereditary diseases.
For the selection of donor-recipient pairs, specialised HSC donor register databases are created. HLA typing of potential donors and entering data into the register is carried out by specialised clinical diagnostic laboratories (J. Filipiak et al., Organization and Development of Bone Marrow Donation and Transplantation in Poland, 2015).
For the creation of registers and selection of donors, it is recommended to carry out typing at 5 loci (HLA-A, HLA-B, HLA-C, HLA-DQB1, HLA-DRB1) at 3-field resolution.
In a clinical setting, HLA genotyping is used to determine the tissue specificity of the donor and recipient to select a histocompatible pair for allogeneic organ or tissue transplantation.
The most common need for finding a donor is to obtain hematopoietic stem cells (HSC) for red bone marrow (RBM) transplantation. HSC transplantation is an effective method for treating oncohaematological and other oncological and hereditary diseases.
For the selection of donor-recipient pairs, specialised HSC donor register databases are created. HLA typing of potential donors and entering data into the register is carried out by specialised clinical diagnostic laboratories (J. Filipiak et al., Organization and Development of Bone Marrow Donation and Transplantation in Poland, 2015).
For the creation of registers and selection of donors, it is recommended to carry out typing at 5 loci (HLA-A, HLA-B, HLA-C, HLA-DQB1, HLA-DRB1) at 3-field resolution.
Purpose
Determination of donor and / or recipient tissue specificity when creating registers and transplanting organs and tissues
Analyzed loci
HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1
Resolution
(3 fields)
Identification of new alleles
Yes
Input
human genomic DNA
Target enrichment
long-range multiplex PCR
Time to results for 192 samples
from 39 to 43 hours
Compatible platforms
Illumina MiSeq
Features
Specifications
Ordering information
GeneValue™ HLA Solution Set A, 192 reactions
Cat.No. GV-HLA-A
Includes: targeted enrichment reagent kit, library preparation kit, molecular barcoding kit (Set A), purification kit, access to the dedicated GeneValue™ HLA Software web application for data analysis
Includes: targeted enrichment reagent kit, library preparation kit, molecular barcoding kit (Set A), purification kit, access to the dedicated GeneValue™ HLA Software web application for data analysis
GeneValue™ HLA Solution Set B, 192 reactions
Cat.No. GV-HLA-B
Includes: targeted enrichment reagent kit, library preparation kit, molecular barcoding kit (Set B), purification kit, access to the dedicated GeneValue™ HLA Software web application for data analysis
Includes: targeted enrichment reagent kit, library preparation kit, molecular barcoding kit (Set B), purification kit, access to the dedicated GeneValue™ HLA Software web application for data analysis